The exhibits come from the excavations conducted in Thessaly during the 20th century up to the present, from offerings and donations of objects as well as from repatriation of antiquities. In total, 2000 objects are exhibited.

The mission of the Museum is to renegotiate the relationship of the people with the past and its remains, as well as to sensitize them towards their protection; this can be established through a dynamic relationship between the people and the terms archaeology, inheritance, culture and culture management. This relationship will be based on the permanent structures of the Museum and will be further enriched by its periodic events.

The main subject of the exhibition is the presentation of the region of Thessaly diachronically, the institutional organization of these societies and the course preceding this organization. The approach towards this course in time and space is achieved through the threefold: state, institutions, authorities. There are distinct subsections in certain sections, which present characteristic issues of the period. These selections were defined by the archaeological material itself.

Specifically the sections are as follows: